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Scientific Project A3 - Initial stages of humus development

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The mechanisms of soil organic matter (SOM) transformation and stabilisation operate on different time scales. We hypothesize that in the initial stage of terrestrial humus formation the selective accumulation of recalcitrant organic matter and the development of hydrophobic surfaces are the dominatin mechanisms. These processes are highly affected by pioneer vegetation development and succession. In the initial phase of ecosystem development, the sources for SOM formation change over time. At long-term scales, stable organo-mineral associations and microaggregation become important after weathering and formation of secondary minerals. Both, the recalcitrance and bioavailability of the accumulating SOM pool are influenced by the formation of organic matter in different soil structures (biogenic crusts, coatings on primary particles, aggregates, pores, rhizosphere).

The subproject A3 intends to identify and quantify relationships between processes of humus formation (origin and quality of organic matter, binding form) and the generation of soil structure (aggregation, hydrophobic surfaces) during initial soil development. The study areas include experimental and reference sites (glacier forefield, sand dunes) and represent initial ecosystems with natural succession (mainly grasses) on sandy substrates.

Parameters indicating the state of soil OM and soil profile development to be determined are:

• degree of development and thickness of soil profiles
• characteristics of structures which store humus (biogenic crusts, aggregates, organic surface layers) and accumulation of free and occluded particulate organic matter
• surface characteristics of mineral particles (specific surface, organic matter loading, wettability)
• chemical composition of organic materials
• amount and composition of organo-mineral associations

For this purpose the following combination of techniques will be applied:

• soil fractionation according to particle size and density to separate plant residues (particulate organic matter) from organo-mineral associations (heavy fractions)
• chemolytic techniques and CPMAS 13C NMR spectroscopy to determine the composition and origin of organic compounds
• characterisation of heavy fractions and sorption experiments of dissolved organic carbon (DOC) to investigate the formation of organo-mineral associations
• FTIR microscopy and synchrotron tomography to scan the location of organic matter stabilisation and to relate chemical and physical properties to specific structures
• determination of degree and persistence of hydrophobicity (effective and potential) to characterise the wettability as parameter for turnover and stabilisation of soil organic matter

After identification of origin, chemistry and types of organic matter accumulation in different stable fractions we deduce the essential processes and structures of humus formation, stabilisation and their change during initial soil development. The results will be used for the dynamic structural and process model to be developed within the framework of SFB/TRR 38.