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Scientific Project B4 - Formation and importance of functional redundancy for microbial nitrogene transformation during initial states of ecosystem development

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Nitrogen is an important factor for the functionality and development of biotic communities in terrestrial ecosystems. Especially in young ecosystems nitrogen is a limiting factor. Thus optimal nitrogen turnover, including an efficient mineralization of organic nitrogen compounds and low denitrification rates are important for a sustainable development of vegetation. Furthermore dinitrogen fixation plays an important role for the additional nitrogen input into the soil. The aim of this subproject is to detect and quantify nitrogen cycle relevant processes on gene, transcript and enzyme levels and to compare it with the respective in situ activities. The main focus is to study the influence of special environmental parameters (e.g. pH, temperature, soil humidity), pioneer plant species and plant communities. The data should be used to deduce the formation and relevance of functional genetic redundancy in the regulation of nitrogen transforming processes in developing ecosystems. The subproject is strongly linked to the subprojects B1 and B3, in order to detect nitrogen and carbon fluxes in the rhizosphere and litter layer of herbaceous plants. The work is directly related to the central hypothesis 1 and 3. The collected data provide the basis for the planned structure and process model (TP C5).